MCQ on Taq Polymerase




Taq DNA polymerase is a heat-resistant enzyme derived from the bacterium Thermus aquaticus. It is widely used in the polymerase chain reaction (PCR) due to its ability to withstand high temperatures, making it suitable for DNA amplification by withstanding the denaturation step at high temperatures during PCR. This is an MCQ on Taq DNA Polymerase and its Significance in PCR, Biotechnology and Genetic Engineering.

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1. 
What is Taq DNA polymerase named after?

2. 
Why is Taq DNA polymerase well-suited for PCR (Polymerase Chain Reaction)?

3. 
At what temperature does Taq DNA polymerase become active in PCR?

4. 
What is the optimal temperature for the extension step in a PCR cycle using Taq DNA polymerase?

5. 
Which property of Taq polymerase makes it different from most other DNA polymerases?

6. 
What is the main role of a DNA primer in a PCR reaction?

7. 
Which step in a PCR cycle involves the addition of nucleotides to the growing DNA strand by Taq polymerase?

8. 
Taq DNA polymerase is used in which molecular biology technique for amplifying DNA?

9. 
Which of the following is a potential drawback of using Taq DNA polymerase in PCR?

10. 
What role do dNTPs (deoxynucleoside triphosphates) play in PCR when Taq polymerase is used?

11. 
Which type of DNA template is suitable for PCR with Taq polymerase?

12. 
Which aspect of the PCR process was significantly enhanced by the use of Taq polymerase?

13. 
Taq polymerase is classified as a thermophilic enzyme. What does this mean?

14. 
In PCR, what is the function of the denaturation step, which typically occurs at a high temperature?

15. 
What is the key advantage of using Taq DNA polymerase in PCR, especially in research and diagnostics?

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Taq Polymerase is Used in PCR because of Its MCQ



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