MCQ on pBR322: Structure and Applications




pBR322 is a well-known plasmid DNA used in molecular biology and biotechnology. It was one of the first plasmids developed for cloning and genetic engineering. pBR322 contains genes for antibiotic resistance and multiple cloning sites, making it a valuable tool for DNA manipulation and recombinant DNA studies. This is an MCQ on pBR322 plasmid – its structure, uses and significance in biotechnology and genetic engineering.

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1. 
What is pBR322?

2. 
In molecular biology, what is the primary purpose of pBR322?

3. 
pBR322 is known for its ability to:

4. 
Which antibiotic resistance genes are commonly found in pBR322?

5. 
In the context of molecular cloning, what is the selective advantage of using pBR322 with antibiotic resistance genes?

6. 
pBR322 contains two origins of replication: one for prokaryotic cells and one for eukaryotic cells. Is this statement true or false?

7. 
In which type of cells is pBR322 primarily replicated?

8. 
Which bacterial species is commonly used for the propagation of pBR322?

9. 
What is the role of the multiple cloning site (MCS) in pBR322?

10. 
What is the term for the process of introducing foreign DNA into pBR322, thereby creating a recombinant plasmid?

11. 
What is the purpose of the tetracycline resistance gene in pBR322?

12. 
Which enzyme is commonly used to cut DNA at specific sites for the purpose of inserting foreign DNA into pBR322?

13. 
What is the name of the process used to transfer the recombinant plasmid (pBR322 with foreign DNA) into bacterial cells?

14. 
Which of the following is NOT a typical use of pBR322 in molecular biology research?

15. 
pBR322 played a significant role in the early development of recombinant DNA technology. Who is credited with its creation?

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MCQ on pBR322



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