MCQ on Illumina Sequencing Technique




Illumina sequencing, a widely used method, employs fluorescently labeled nucleotides and reversible termination to sequence DNA fragments. Through iterative cycles of nucleotide incorporation and imaging, it accurately determines the sequence, offering high throughput, speed, and cost-effectiveness, revolutionizing genomic research and applications. This is an MCQ on Illumina Sequencing Technique of whole genome sequencing. 

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1. 
What is the primary principle behind Illumina dye sequencing?

2. 
Which enzyme is used during Illumina dye sequencing to incorporate fluorescently labeled nucleotides?

3. 
What is the purpose of reversible terminators in Illumina sequencing?

4. 
Which term describes the process where one nucleotide is added at a time during Illumina sequencing?

5. 
What role do fluorescently labelled nucleotides play in Illumina sequencing?

6. 
In Illumina sequencing, what distinguishes the different nucleotides from one another?

7. 
Which color-coding scheme is typically used for the four different nucleotides in Illumina sequencing?

8. 
How are fluorescent signals detected in Illumina sequencing?

9. 
What does the term "cluster generation" refer to in Illumina sequencing?

10. 
What role do the adapters serve in Illumina library preparation?

11. 
Which step in Illumina sequencing involves the removal of the fluorescent label after imaging?

12. 
What is the primary advantage of using reversible terminators in Illumina sequencing?

13. 
Which term describes the process where clusters of identical DNA sequences are generated during Illumina sequencing?

14. 
How does Illumina sequencing achieve high-throughput sequencing?

15. 
What is the primary factor that contributes to the accuracy of Illumina sequencing?

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mcq on illumina sequencing



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