MCQ on CRISPR Cas-9: Principles and Applications




The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins) system is a revolutionary genome editing tool in molecular biology. It enables precise modification of DNA sequences in living organisms, allowing for gene insertion, deletion, or replacement. Cas proteins, guided by RNA molecules, target specific DNA sequences for modification, offering great potential in various scientific and medical applications. This is an MCQ on CRISPR-CAS and its applications in biological research.

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Learn more: MCQ on Cre-Lox Recombination

1. 
What does "CRISPR" stand for in the context of genome editing?

2. 
What is the primary function of the CRISPR-Cas system in bacteria?

3. 
Which enzyme is commonly used in the CRISPR-Cas9 system to cut DNA at specific locations?

4. 
In the CRISPR-Cas9 system, what molecule guides Cas9 to the target DNA sequence?

5. 
Which of the following is NOT one of the key components of the CRISPR-Cas9 system?

6. 
What is the role of the guide RNA (gRNA) in the CRISPR-Cas9 system?

7. 
In the CRISPR-Cas system, how does Cas9 create a double-strand break in the DNA?

8. 
What term is used to describe the repair mechanism that can occur after a DNA double-strand break caused by the CRISPR-Cas9 system?

9. 
What is the primary advantage of the CRISPR-Cas9 system over earlier genome editing techniques like zinc finger nucleases and TALENs?

10. 
Which application of the CRISPR-Cas system allows for the correction of genetic mutations responsible for inherited diseases?

11. 
Which Cas protein is commonly used for base editing in the CRISPR-Cas system, enabling the conversion of one DNA base pair into another?

12. 
What does "Cas" stand for in the CRISPR-Cas system?

13. 
Which organism was the source of the CRISPR-Cas system that has been widely used for genome editing?

14. 
What are some ethical concerns associated with the CRISPR-Cas system?

15. 
In addition to gene editing, what other applications has the CRISPR-Cas system found use in?

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  3. MCQ on pBR322: Structure and Applications
  4. MCQ on Taq Polymerase
  5. MCQ on Genetic Markers

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CRISPR Cas-9 MCQ




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Related posts:

  1. MCQ on Cre-Lox Recombination: Principles and Applications
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  3. MCQ on pBR322: Structure and Applications
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