MCQ on Shotgun Sequencing




Shotgun sequencing is a method to sequence DNA by randomly breaking it into smaller fragments, sequencing them, and then assembling the overlapping sequences. This approach enables rapid determination of the entire genome, facilitating genomic research and genome assembly. This is an MCQ on Shotgun Sequencing Methodology of DNA Sequencing for Whole Genome Sequencing Studies.

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1. 
What is the primary goal of shotgun sequencing?

2. 
In shotgun sequencing, DNA is fragmented into:

3. 
Which technology allows for the random fragmentation of DNA in shotgun sequencing?

4. 
What is the purpose of assembling overlapping DNA fragments in shotgun sequencing?

5. 
Which term describes the step where overlapping sequences are aligned and merged to reconstruct the entire genome in shotgun sequencing?

6. 
In shotgun sequencing, what technology is commonly used to determine the sequence of individual DNA fragments?

7. 
How are overlapping DNA fragments identified and aligned during shotgun sequencing?

8. 
Which aspect makes shotgun sequencing advantageous for whole-genome sequencing?

9. 
What is the primary challenge in shotgun sequencing?

10. 
Which term refers to the random distribution of DNA fragments in shotgun sequencing?

11. 
What is the purpose of quality control in shotgun sequencing?

12. 
Which step precedes DNA sequencing in shotgun sequencing?

13. 
Which sequencing approach is primarily associated with the concept of "shotgun" sequencing?

14. 
What is the advantage of non-overlapping fragments in shotgun sequencing?

15. 
Which term describes the computational process of reconstructing the original sequence from overlapping fragments in shotgun sequencing?

Related posts:

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  2. MCQ on Pyrosequencing of DNA
  3. MCQ on Southern Blotting Principles and Applications
  4. MCQ on Taq Polymerase
  5. MCQ on EMSA (Electrophoretic Mobility Shift Assay)

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MCQ on Shotgun Sequencing



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Related posts:

  1. MCQ on Illumina Sequencing Technique
  2. MCQ on Pyrosequencing of DNA
  3. MCQ on Southern Blotting Principles and Applications
  4. MCQ on Taq Polymerase
  5. MCQ on EMSA (Electrophoretic Mobility Shift Assay)

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