MCQ on EMSA (Electrophoretic Mobility Shift Assay)




Electrophoretic Mobility Shift Assay (EMSA), also known as gel shift or band shift assay, is a molecular biology technique. It’s used to study protein–DNA interactions by observing changes in the mobility of DNA fragments in an electrophoresis gel when proteins bind to them, helping analyze DNA-protein interactions. This is an MCQ on EMSA Principles and Applications.

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1. 
What is the primary purpose of EMSA?

2. 
In EMSA, what is typically labeled to visualize the DNA-protein complexes?

3. 
complex with a protein in EMSA?

4. 
Which step of EMSA involves mixing the DNA probe with nuclear or cell extracts containing the protein of interest?

5. 
What is the purpose of the gel electrophoresis step in EMSA?

6. 
In EMSA, what is the term for a DNA fragment that has migrated through the gel without forming a complex with a protein?

7. 
What is the key advantage of using a radioactive probe in EMSA?

8. 
What is the role of the unlabeled competitor DNA in EMSA?

9. 
Which type of gel is commonly used in EMSA?

10. 
What is the primary function of the protein-DNA binding buffer in EMSA?

11. 
Which of the following is NOT a common application of EMSA?

12. 
In EMSA, what happens to the mobility of DNA-protein complexes when the protein-DNA binding is strong?

13. 
What is the primary purpose of protein denaturation in EMSA?

14. 
Which step in EMSA is critical for distinguishing specific DNA-protein complexes from non-specific interactions?

15. 
In EMSA, what is the primary purpose of the autoradiography or fluorescent imaging step?

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MCQ on EMSA Principles and Applications




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