MCQ on DNA Sequencing




DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It provides crucial insights into genetic makeup, aiding in various fields like medicine, agriculture, and evolutionary biology by understanding genetic traits, mutations, and relationships. This is an MCQ on DNA Sequencing Principles and its Applications in Genetic Engineering and Genomics.

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1. 
What is the primary goal of DNA sequencing?

2. 
Which technique is commonly used for high-throughput DNA sequencing?

3. 
What is the role of DNA polymerase in DNA sequencing?

4. 
Which nucleotide is used as a 'terminator' in Sanger sequencing?

5. 
What does PCR stand for in the context of DNA sequencing?

6. 
Which sequencing technique involves the use of fluorescently labeled nucleotides?

7. 
What is the purpose of library preparation in DNA sequencing?

8. 
Which component of DNA sequencing technology reads the sequence of bases in the DNA template?

9. 
Which DNA sequencing method provides long-read sequencing capabilities?

10. 
What is the key advantage of NGS over Sanger sequencing?

11. 
Which step is crucial in the preparation of DNA samples for sequencing?

12. 
What is the purpose of base calling in DNA sequencing?

13. 
Which sequencing technology utilizes the synthesis of DNA by detecting released pyrophosphate?

14. 
What does the term "shotgun sequencing" refer to in DNA sequencing?

15. 
Which sequencing approach is commonly used in whole-genome sequencing projects?

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MCQ on DNA Sequencing



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