MCQ on Immunofluorescence: Principles and Applications




Immunofluorescence is a laboratory technique that utilizes fluorescently labeled antibodies to detect and visualize specific proteins or antigens within cells or tissues. When exposed to light of a particular wavelength, the labeled antibodies emit fluorescence, enabling precise localization and identification of target molecules in biological samples. This is an MCQ on Immunofluorescence – Principles and Applications.

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Learn more: MCQ on Green Fluorescent Protein

1. 
What is the primary purpose of immunofluorescence?

2. 
In immunofluorescence, what molecules are labeled with fluorescent dyes or antibodies for detection?

3. 
What is the term for the process of binding a fluorescent molecule to a specific antibody or antigen?

4. 
What is the primary role of the secondary antibody in immunofluorescence?

5. 
In direct immunofluorescence, what directly binds to the antigen of interest in the sample?

6. 
What is the role of the mounting medium in immunofluorescence?

7. 
Which of the following is commonly used to counterstain the nucleus in immunofluorescence?

8. 
In indirect immunofluorescence, what does the secondary antibody recognize and bind to?

9. 
What is the primary purpose of the wash steps in immunofluorescence?

10. 
Which of the following is NOT a commonly used fluorophore in immunofluorescence?

11. 
In immunofluorescence, what color does a fluorophore emit when it is excited by a specific wavelength of light?

12. 
Which type of immunofluorescence is often used for detecting cell surface antigens and is commonly seen in flow cytometry?

13. 
What is the purpose of the incubation step with antibodies in immunofluorescence?

14. 
Which part of the microscope is used to filter and select the specific wavelength of light for excitation in immunofluorescence?

15. 
What is the main advantage of using immunofluorescence in research and diagnostics?

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MCQ on Immunofluorescence




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Related posts:

  1. MCQ on Green Fluorescent Protein (GFP)
  2. MCQ on Cre-Lox Recombination: Principles and Applications
  3. MCQ on CRISPR Cas-9: Principles and Applications
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