MCQ on RIA (Radioimmunoassay)




Radioimmunoassay (RIA) is a sensitive laboratory technique that uses radioactive isotopes to measure the concentration of specific substances, such as hormones or antigens, in biological samples. It involves competition between labeled and unlabeled molecules for binding to a limited number of specific antibodies. This is an MCQ on RIA (Radioimmunoassay)- Principles and Applications.

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1. 
What is the primary purpose of Radioimmunoassay (RIA)?

2. 
In RIA, what component is labeled with a radioactive isotope for detection?

3. 
What is the role of the standard curve in RIA?

4. 
Which radioactive isotope is commonly used in RIA?

5. 
In a competitive RIA, what competes with the sample antigen for binding to the antibody?

6. 
What is the primary purpose of the separation step in RIA?

7. 
In non-competitive RIA, what is labeled with a radioactive isotope?

8. 
What is the term for the substance used to block any remaining binding sites on the solid phase in RIA?

9. 
What is the purpose of the scintillation fluid in RIA?

10. 
Which of the following is NOT a commonly used radioisotope in RIA?

11. 
What type of binding does competitive RIA measure?

12. 
In RIA, what is the name of the container that holds the solid phase where the antigen-antibody complexes are formed?

13. 
What is the purpose of using scintillation counting in RIA?

14. 
What is the main advantage of using RIA over other immunoassays, such as ELISA?

15. 
What is the primary application of RIA in the field of medicine?

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MCQ on RIA




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