MCQ on Western Blotting Technique




Western blotting is a laboratory technique used to detect and analyze proteins. It involves separating proteins based on size through gel electrophoresis, transferring them to a membrane, and then using specific antibodies to visualize and identify the target proteins. This is an MCQ on Western Blotting – Principles and Applications

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1. 
What is the primary purpose of Western blotting?

2. 
Who coined the term Western blotting?

3. 
In Western blotting, what is the first step in the process?

4. 
Which type of molecules is separated by size in a Western blot?

5. 
After separation by size in SDS-PAGE, how are proteins transferred to a solid support in Western blotting?

6. 
What is the solid support typically used in Western blotting to immobilize proteins?

7. 
What is the purpose of the blotting step in Western blotting?

8. 
What is the primary purpose of blocking in Western blotting?

9. 
What is the probe in Western blotting?

10. 
What is the role of the probe in Western blotting?

11. 
How is the target protein visualized after incubation with the probe in Western blotting?

12. 
Which of the following is NOT an application of Western blotting?

13. 
Which of the following is an essential component for the detection step in Western blotting?

14. 
In Western blotting, what is the role of chemiluminescence or colorimetric detection?

15. 
Which blotting technique is used for DNA molecules, as opposed to proteins?

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MCQ on Western Blotting




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Related posts:

  1. MCQ on Immunofluorescence: Principles and Applications
  2. MCQ on Green Fluorescent Protein (GFP)
  3. MCQ on Cre-Lox Recombination: Principles and Applications
  4. MCQ on EMSA (Electrophoretic Mobility Shift Assay)
  5. MCQ on Northern Blotting: Principles and Applications

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