MCQ on CRISPR-Cas9 System

1. 
What does "CRISPR" stand for in the context of genome editing?

2. 
What is the primary function of the CRISPR-Cas system in bacteria?

3. 
Which enzyme is commonly used in the CRISPR-Cas9 system to cut DNA at specific locations?

4. 
In the CRISPR-Cas9 system, what molecule guides Cas9 to the target DNA sequence?

5. 
Which of the following is NOT one of the key components of the CRISPR-Cas9 system?

6. 
What is the role of the guide RNA (gRNA) in the CRISPR-Cas9 system?

7. 
In the CRISPR-Cas system, how does Cas9 create a double-strand break in the DNA?

8. 
What term is used to describe the repair mechanism that can occur after a DNA double-strand break caused by the CRISPR-Cas9 system?

9. 
What is the primary advantage of the CRISPR-Cas9 system over earlier genome editing techniques like zinc finger nucleases and TALENs?

10. 
Which application of the CRISPR-Cas system allows for the correction of genetic mutations responsible for inherited diseases?

11. 
Which Cas protein is commonly used for base editing in the CRISPR-Cas system, enabling the conversion of one DNA base pair into another?

12. 
What does "Cas" stand for in the CRISPR-Cas system?

13. 
Which organism was the source of the CRISPR-Cas system that has been widely used for genome editing?

14. 
What are some ethical concerns associated with the CRISPR-Cas system?

15. 
In addition to gene editing, what other applications has the CRISPR-Cas system found use in?



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