Purines are nitrogenous organic compounds that consist of a double-ring structure. They are essential components of DNA and RNA and play crucial roles in cellular processes. Common purines include adenine and guanine, which are involved in genetic information storage and various metabolic functions. This is an MCQ on Purines: Structure […]
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MCQ on Taq Polymerase
Taq DNA polymerase is a heat-resistant enzyme derived from the bacterium Thermus aquaticus. It is widely used in the polymerase chain reaction (PCR) due to its ability to withstand high temperatures, making it suitable for DNA amplification by withstanding the denaturation step at high temperatures during PCR. This is an […]
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MCQ on Southern Blotting Principles and Applications
Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample. It involves digesting DNA, electrophoresis, transferring the DNA to a membrane, and then using a labeled probe to hybridize and identify the target DNA sequence. This technique is named after its inventor, Edwin Southern. […]
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MCQ on pBR322: Structure and Applications
pBR322 is a well-known plasmid DNA used in molecular biology and biotechnology. It was one of the first plasmids developed for cloning and genetic engineering. pBR322 contains genes for antibiotic resistance and multiple cloning sites, making it a valuable tool for DNA manipulation and recombinant DNA studies. This is an […]
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MCQ on Pyrimidine Synthesis
Pyrimidine synthesis is a biochemical pathway to produce pyrimidine nucleotides, which are key components of DNA, RNA, and molecules like ATP and UTP. It involves a series of enzymatic reactions that convert precursor molecules into pyrimidines, such as cytosine, thymine, and uracil, essential for genetic and metabolic processes. This is […]
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