MCQ on Gene Knockout Technology- Principles and Applications




Gene knockout is a genetic technique wherein a specific gene is intentionally deactivated or “knocked out” in an organism. This allows researchers to study the effects of gene loss, revealing insights into gene function and its role in biological processes. This is an MCQ on Gene Knockout Technology- Principles and Applications

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1. 
What is the primary purpose of genetic knockout in biological research?

2. 
Which term best describes the resulting organisms or cells after a successful genetic knockout?

3. 
What is the main difference between gene knockout and gene knockdown techniques?

4. 
Which type of organism is often used in genetic knockout studies due to its well-characterized genetics and relatively short generation time?

5. 
What is the primary method for achieving genetic knockout in animals like mice?

6. 
What is the result of a heterozygous genetic knockout?

7. 
In a homozygous genetic knockout, what is the genetic status of the target gene?

8. 
What is the main purpose of generating knockout mice in research?

9. 
Which of the following is NOT a method for creating genetic knockouts in mice?

10. 
What is the primary goal of using RNAi in genetic knockout studies?

11. 
In plants, which technique is commonly used to generate genetic knockouts by reducing gene expression?

12. 
Which term is used to describe a genetic knockout that affects the expression of a target gene in a specific tissue or at a specific developmental stage?

13. 
What is the primary advantage of conditional knockout techniques in genetic research?

14. 
In the context of genetic knockout, what is the purpose of creating a "knockdown" instead of a complete knockout?

15. 
What are the potential ethical considerations in genetic knockout studies?

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MCQ on Gene Knockout Technology




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  4. MCQ on EMSA (Electrophoretic Mobility Shift Assay)
  5. MCQ on Northern Blotting: Principles and Applications

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