MCQ on EMSA Principles and Applications

MCQ on EMSA (Electrophoretic Mobility Shift Assay)

Electrophoretic Mobility Shift Assay (EMSA), also known as gel shift or band shift assay, is a molecular biology technique. It’s used to study protein–DNA interactions by observing changes in the mobility of DNA fragments in an electrophoresis gel when proteins bind to them, helping analyze DNA-protein interactions. This is an […]

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MCQ on ELISA

MCQ on ELISA (Enzyme Linked Immuno Sorbent Assay)

Enzyme-Linked Immunosorbent Assay (ELISA) is a biochemical test used to detect and quantify specific proteins, antibodies, or antigens in biological samples. It involves using antibodies and enzymes to create a color change that indicates the presence and concentration of the target molecule, making it valuable in various research and diagnostic […]

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CRISPR Cas-9 MCQ

MCQ on CRISPR Cas-9: Principles and Applications

The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins) system is a revolutionary genome editing tool in molecular biology. It enables precise modification of DNA sequences in living organisms, allowing for gene insertion, deletion, or replacement. Cas proteins, guided by RNA molecules, target specific DNA sequences for modification, […]

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MCQ on Cre Lox Recombination

MCQ on Cre-Lox Recombination: Principles and Applications

Cre-lox recombination is a genetic engineering technique used to manipulate DNA in living organisms. It involves the enzyme Cre recombinase, which recognizes specific DNA sequences (loxP sites) to excise, insert, or invert genetic segments. It is valuable for conditional gene knockout and controlled gene expression in research and biotechnology. This […]

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Taq Polymerase is Used in PCR because of Its MCQ

MCQ on Taq Polymerase

Taq DNA polymerase is a heat-resistant enzyme derived from the bacterium Thermus aquaticus. It is widely used in the polymerase chain reaction (PCR) due to its ability to withstand high temperatures, making it suitable for DNA amplification by withstanding the denaturation step at high temperatures during PCR. This is an […]

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