Taq DNA polymerase is a heat-resistant enzyme derived from the bacterium Thermus aquaticus. It is widely used in the polymerase chain reaction (PCR) due to its ability to withstand high temperatures, making it suitable for DNA amplification by withstanding the denaturation step at high temperatures during PCR. This is an […]
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MCQ on Southern Blotting Principles and Applications
Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample. It involves digesting DNA, electrophoresis, transferring the DNA to a membrane, and then using a labeled probe to hybridize and identify the target DNA sequence. This technique is named after its inventor, Edwin Southern. […]
Continue ReadingMCQ on pBR322: Structure and Applications
pBR322 is a well-known plasmid DNA used in molecular biology and biotechnology. It was one of the first plasmids developed for cloning and genetic engineering. pBR322 contains genes for antibiotic resistance and multiple cloning sites, making it a valuable tool for DNA manipulation and recombinant DNA studies. This is an […]
Continue ReadingMCQs on TI Plasmid and its Applications in Biotechnology
A TI (tumour-inducing) plasmid is a type of circular DNA molecule found in certain strains of the bacterium Agrobacterium tumefaciens. It plays a crucial role in the bacterium’s ability to infect plants and transfer genes into them, causing the formation of crown gall tumours. TI plasmids have been extensively studied […]
Continue ReadingMCQ on DNA Barcoding and its Applications
DNA barcoding is a molecular technique that uses a short, standardized DNA sequence from a specific gene to identify and classify species. It helps scientists and researchers accurately distinguish and catalogue organisms, aiding in biodiversity research, conservation, and various scientific applications. This post is on applications of DNA barcoding MCQ […]
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