pBR322 is a well-known plasmid DNA used in molecular biology and biotechnology. It was one of the first plasmids developed for cloning and genetic engineering. pBR322 contains genes for antibiotic resistance and multiple cloning sites, making it a valuable tool for DNA manipulation and recombinant DNA studies. This is an […]
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MCQs on TI Plasmid and its Applications in Biotechnology
A TI (tumour-inducing) plasmid is a type of circular DNA molecule found in certain strains of the bacterium Agrobacterium tumefaciens. It plays a crucial role in the bacterium’s ability to infect plants and transfer genes into them, causing the formation of crown gall tumours. TI plasmids have been extensively studied […]
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Applications of Restriction Endonucleases in Biotechnology (Short Notes)
Restriction Endonucleases Restriction endonucleases (also called as molecular scissors) are a class of nuclease enzymes which cut the DNA strand at precise locations. They are specific endonuclease enzymes in the cells which first recognize the specific sequence (called restriction sites) within the DNA strand and cleave the phosphodiester backbone of […]
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