Phase Contrast Microscope vs Differential Interference Contrast Microscope
(Similarities and Differences between Phase Contrast Microscope and Differential Interference Contrast (DIC) Microscope)
Phase contrast microscopy and Differential Interference Contrast (DIC) microscopy are two advanced optical light microscopy techniques to produce high contrast images of unstained and living cells. Both the microscopes utilize various contrast enhancing techniques to produce high contrast images.
Phase contrast microscopy is an optical-microscopy technique developed by Frits Zernike in 1934 to produce high contrast images of unstained live specimens. The phase contrast microscopy works by converting the phase shifts of light passing through a transparent specimen to detectable brightness changes in the image.
Differential Interference Contrast (DIC) microscopy, also called as Nomarski Interference Contrast (NIC) Microscopy, was first invented by Georges Nomarski in 1952. DIC microscopy uses more sophisticated contrast enhancing techniques than phase contrast system. It works by separating a polarized light source into two orthogonally polarized mutually coherent parts which are spatially displaced at the sample plane, and recombined before the final image formation. DIC produce more pronounced contrast difference than phase contrast image.