Biology MCQ (Multiple Choice Questions in Life Science)
(Sample/Model/Practice Questions for JRF/NET Life Science Examination, ICMR JRF, DBT JRF, GATE, ICAR NET, PG Entrance)
Biophysical Instrumentation: Separation Techniques Part 1: (MCQ 009)
1). When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as:
a. E = V/d
b. E = (1/V) x q
c. E = (Vd)/q
d. E = V + d
2). The velocity (‘v’) of a charged particles in an electric field in a medium can be mathematically expressed as v = Eq/f, where ‘Eq’ and ‘f’ are ________.
a. Eq: Energy; f: Frictional force
b. Eq: Electrical force; f: Gravitational force
c. Eq: Electrical force; f: Frictional co-efficient
d. Eq: Equilibrium constant; f: co-efficient of gravity
3). For the separation of DNA by electrophoresis, which of the following method is commonly used?
a. Agarose – vertical
b. Agarose – horizontal
c. PAGE – vertical
d. PAGE – horizontal
4). Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________.
a. An anionic detergent
b. A cationic detergent
c. A non-ionic detergent
d. An anion exchanger
e. A cation exchanger
5). Function of β-mercaptoethanol in SDS-PAGE is__________.