Difference between Gas Solid Chromatography and Gas Liquid Chromatography: Comparison Table

Difference between GSC and GLC

Gas-Solid Chromatography (GSC) vs Gas-Liquid Chromatography (GLC)
(Difference between GSC and GLC Chromatography)


Gas Chromatography or GC is a chromatographic technique used for the separation of volatile compounds. In GC, a mixture of volatile compounds with differential migration passes through a column containing solid or liquid stationary phase. In GC, the mixture to be separated should be in gaseous phase and the mobile phase used will be always in the gaseous state (usually an inert gas such as nitrogen, helium or argon). Based on the states of stationary phases, there are two types of Gas Chromatography techniques: (1) Gas Solid Chromatography (GSC) and (2) Gas Liquid Chromatography (GLC).

(1). Gas Solid Chromatography (GSC):

In GSC, the stationary phase is in the solid state (liquid phase is absent). The stationary phase is coated in the interior of the column. Molecules in the stationary phase will interact with the mobile phase through adsorption forces.

(2). Gas Liquid Chromatography (GLC):

In GLC, the stationary phase is a nonvolatile liquid. The liquid stationary phase is coated on an inert support in the column. The mobile phase will be an inert gas such as nitrogen, helium or argon.

There are considerable differences in the working principle, mobile and stationary phase and the efficiency of GSC and GLC. The present post discusses the similarities and differences between Gas Solid Chromatography (GSC) and Gas Liquid Chromatography (GLC) with a comparison table.

Similarities between Gas Liquid and Gas Solid Chromatography

Ø  Both are GSC and GLC are Gas chromatographic techniques.

Ø  Both uses gaseous mobile phase (usually an inert gas such as N, He etc.).

Ø  Both are used for the separation of volatile compounds / mixtures.

Ø  Heat labile compounds cannot be separated.

Ø  Both GSC and GLC can be analytic or preparatory.

Ø  Both types of GC use similar type of detectors.

Continue reading

mcq biology

Biophysics MCQ: Electrophoresis: for JRF NET Life Science Examination (Biology MCQ 009): Biophysical Instrumentation: Separation Techniques Part 1

Biophysics Quizzes

Biology MCQ (Multiple Choice Questions in Life Science)
(Sample/Model/Practice Questions for JRF/NET Life Science Examination, ICMR JRF, DBT JRF, GATE, ICAR NET, PG Entrance)

Biophysical Instrumentation: Separation Techniques Part 1: (MCQ 009)

1). When voltage ‘V’ is applied across a pair of electrode (cathode and anode), a potential gradient ‘E’ is created between the electrodes. We can calculate ‘E’ as:

a.      E = V/d
b.      E = (1/V) x q
c.       E = (Vd)/q
d.      E = V + d

2). The velocity (‘v’) of a charged particles in an electric field in a medium can be mathematically expressed as v = Eq/f, where ‘Eq’ and ‘f’ are ________.

a.      Eq: Energy; f: Frictional force
b.      Eq: Electrical force; f: Gravitational force
c.       Eq: Electrical force; f: Frictional co-efficient
d.      Eq: Equilibrium constant; f: co-efficient of gravity

3). For the separation of DNA by electrophoresis, which of the following method is commonly used?

a.      Agarose – vertical
b.      Agarose – horizontal
c.       PAGE – vertical
d.      PAGE – horizontal

4). Sodium dodecyl sulfate (SDS) used in SDS PAGE is___________.

a.      An anionic detergent
b.      A cationic detergent
c.       A non-ionic detergent
d.      An anion exchanger
e.       A cation exchanger

5). Function of β-mercaptoethanol in SDS-PAGE is__________.

Continue reading