MCQ on Genetic Engineering (Biotechnology MCQ-05) Lecture Notes MCQ Biology Welcome to Biotechnology MCQ 05 Genetic Engineering (Advanced) This question set on Biotechnology MCQ-05: Genetic Engineering (Advanced) consists of 15 questions in MCQ format. Please select the correct answer and at the end of the test, you have to click on 'SUBMIT' button to see your Score and the Correct Answers. Please click 'NEXT' button to start the quiz... 1. ‘Taq’ enzyme utilized in PCR is a: DNA polymerase without 3' to 5' exonuclease proofreading activity DNA polymerase without 5' to 3' exonuclease proofreading activity DNA polymerase with 3' to 5' exonuclease proofreading activity DNA polymerase with 5' to 3' exonuclease proofreading activity2. Which of the following is an RNA blotting technique? Western blotting Eastern blotting Northern blotting Southern blotting3. The enzyme used in homopolymer tailing in recombinant DNA technology is: Terminal nucleotide synthase Terminal deoxy nucleotidyltransferase Terminal DNA polymerase Terminal dT synthase4. The most common restriction endonuclease enzyme used in the recombination DNA technology is: Type IV restriction endonucleases Type III restriction endonucleases Type II restriction endonuclease Type I restriction endonuclease5. When a linear DNA segment is treated with the alkaline phosphatase enzyme, which of the following is likely to occur: The enzyme add a phosphate group to 5’ terminal The enzyme removes the 3’ terminal phosphate group The enzyme add a phosphate group to 3’ terminal The enzyme removes the 5’ terminal phosphate group6. A vector that is specifically designed to replicate in two different hosts are referred to as: Dual vector Multiple host vector Shuttle vector High fidelity vector7. The best system which can be adopted for gene therapy is__________. Ac-Ds elements P elements Non-homogenous recombination Cre-Lox system8. The enzyme used to hydrolyze RNA from an RNA-DNA heteroduplex is: RNase A RNase C RNase B RNase H9. The source of dwarfing genes in wheat is____ Ganga 101 Dee-geo-woo-gen Norin 10 Sonalika10. The co-factor required for the E. coli DNA ligase enzyme is: ATP FAD⁺ NAD⁺ GTP11. Which of the following vectors can carry the longest piece of foreign DNA? Plasmids Bacteriophage Cosmids Yeast artificial chromosome (YAC)12. The enzyme used to prevent the unwanted ligation of DNA molecules during cloning is: Terminal peroxidase Horse Radish Peroxidase (HRP) Alkaline phosphatase Terminal phosphatase13. Which of the following organism has been extensively used in the generation of transgenic plants? Agrobacterium tumefaciens Baculoviruses Bacilus thuringensis E. coli14. When a linear DNA is treated with the enzyme polynucleotide kinase, which of the following is likely to occur: The enzyme adds phosphate from GTP to the 5’-OH of the DNA The enzyme adds phosphate from ATP to the 5’-OH of the DNA. The enzyme removes nucleotides from the 5’ end. The enzyme adds ATP to the 5’ end of the DNA15. The co-factor required for the T4 DNA ligase enzyme is: NAD⁺ GTP ATP FAD⁺You have reached at the End of this Quiz. Please Click 'SUBMIT' button to see your Score and the Correct Answers... Get our Updates on BIOTECHNOLOGY in your E-mail Inbox We will not spam your account… Enter your e-mail address Don’t forget to Activate your Subscription…. 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