MCQ on Genetic Engineering (Biotechnology MCQ-05) Lecture Notes MCQ Biology Welcome to Biotechnology MCQ 05 Genetic Engineering (Advanced) This question set on Biotechnology MCQ-05: Genetic Engineering (Advanced) consists of 15 questions in MCQ format. Please select the correct answer and at the end of the test, you have to click on 'SUBMIT' button to see your Score and the Correct Answers. Please click 'NEXT' button to start the quiz... 1. The enzyme used in homopolymer tailing in recombinant DNA technology is: Terminal DNA polymerase Terminal deoxy nucleotidyltransferase Terminal dT synthase Terminal nucleotide synthase2. The co-factor required for the E. coli DNA ligase enzyme is: ATP GTP FAD⁺ NAD⁺3. The enzyme used to prevent the unwanted ligation of DNA molecules during cloning is: Alkaline phosphatase Horse Radish Peroxidase (HRP) Terminal phosphatase Terminal peroxidase4. The co-factor required for the T4 DNA ligase enzyme is: GTP ATP NAD⁺ FAD⁺5. The source of dwarfing genes in wheat is____ Sonalika Ganga 101 Norin 10 Dee-geo-woo-gen6. A vector that is specifically designed to replicate in two different hosts are referred to as: Dual vector Shuttle vector High fidelity vector Multiple host vector7. The enzyme used to hydrolyze RNA from an RNA-DNA heteroduplex is: RNase A RNase C RNase B RNase H8. ‘Taq’ enzyme utilized in PCR is a: DNA polymerase with 5' to 3' exonuclease proofreading activity DNA polymerase without 5' to 3' exonuclease proofreading activity DNA polymerase with 3' to 5' exonuclease proofreading activity DNA polymerase without 3' to 5' exonuclease proofreading activity9. The best system which can be adopted for gene therapy is__________. Ac-Ds elements Non-homogenous recombination P elements Cre-Lox system10. Which of the following organism has been extensively used in the generation of transgenic plants? Baculoviruses Bacilus thuringensis Agrobacterium tumefaciens E. coli11. When a linear DNA segment is treated with the alkaline phosphatase enzyme, which of the following is likely to occur: The enzyme add a phosphate group to 3’ terminal The enzyme removes the 5’ terminal phosphate group The enzyme removes the 3’ terminal phosphate group The enzyme add a phosphate group to 5’ terminal12. The most common restriction endonuclease enzyme used in the recombination DNA technology is: Type II restriction endonuclease Type IV restriction endonucleases Type I restriction endonuclease Type III restriction endonucleases13. When a linear DNA is treated with the enzyme polynucleotide kinase, which of the following is likely to occur: The enzyme adds phosphate from ATP to the 5’-OH of the DNA. The enzyme removes nucleotides from the 5’ end. The enzyme adds phosphate from GTP to the 5’-OH of the DNA The enzyme adds ATP to the 5’ end of the DNA14. Which of the following vectors can carry the longest piece of foreign DNA? Bacteriophage Cosmids Plasmids Yeast artificial chromosome (YAC)15. Which of the following is an RNA blotting technique? Western blotting Southern blotting Northern blotting Eastern blottingYou have reached at the End of this Quiz. Please Click 'SUBMIT' button to see your Score and the Correct Answers... Get our Updates on BIOTECHNOLOGY in your E-mail Inbox We will not spam your account… Enter your e-mail address Don’t forget to Activate your Subscription…. 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Please leave me in the Comments Section below. I will be Happy to Read your Comments and Reply. Please Share with your Friends, Relatives, Students and Colleagues… Related posts: DNA Recombination Technology MCQ with Answer Key (Biotechnology MCQ 04) MCQ on Industrial Biotechnology with Answer Key (Biotechnology MCQ 03) Biotechnology MCQ 01 with Answer Key and Explanations MCQ on Genetic Engineering with Answer Key (MCQ 02) Posted in Biology MCQ, Biotechnology MCQ, Genetic Engineering, rDNA Technology and tagged Biotechnology MCQ with Answer Key, Biotechnology MCQ with Answers, Biotechnology Mock Test, Biotechnology Online Test, Biotechnology Practice Questions.