HSST Botany Junior & Senior Kerala PSC Question Paper with Answer Key and Explanations: Part I (Q 1 – 25)

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HSST Botany Previous Year Question Papers

Kerala PSC HSST Botany 2012 Examination
Botany Higher Secondary School Teacher: Junior & Senior
Question Paper Code 40/2012 (Cat. NO. 449/2010)

Original question paper of Kerala PSC HSST Botany Junior / Senior (Higher Secondary School Teacher Botany), Category No. 449/2010) examination conducted by Kerala PSC (Public Service Commission) on 13/04/2002 (Q. Code 40/2012) for the appointment of HSST Botany in Government Higher Secondary Schools of Kerala under the Directorate of Higher Secondary Education, Trivandrum, Govt. of Kerala. Questions are in MCQ (Multiple Choice Questions) format.

Part – 1 (Questions 1 – 25)


(1). Key enzyme in PCR:

a.       Taq polymerase
b.      EcoRI
c.       Restriction endonuclease
d.      Ligase

Ans. (a). Taq polymerase

Taq DNA polymerase is a thermostable DNA polymerase enzyme obtained from a thermophilic bacterium Thermus aquaticus. Taq DNA polymerase is frequently used polymerase enzyme in Polymerase Chain Reaction (PCR). The optimum temperature for Taq DNA polymerase is 72oC. Taq DNA polymerase does not have the 3’ – 5’ exonuclease proof reading activity and thus the fidelity of this enzyme is relatively low. Taq DNA polymerase adds a 3’ ‘A’ (adenine) overhang at the 3’ end of PCR products. This property can be used in the ‘TA’ cloning procedure where the PCR products can be easily ligated (cloned) to a vector containing ‘T’ overhangs.

Pfu polymerase is another thermostable DNA polymerase used in PCR, isolated from the bacterium Pyrococcus furiosus. The advantage of using Pfu polymerase over Taq polymerase is that, Pfu polymerase enzyme processes the 3’ – 5’ exonuclease proof reading activity and hence this enzyme shows high fidelity.

EcoRI: restriction enzymes obtained from E. coli.

Restriction enzyme: also called as molecular scissors, are endonuclease enzymes with cut DNA on specific sites, they are important tool in genetic engineering.

Ligase: a class of enzyme which joins two molecules by the formation of new covalent bonds between the two molecules with energy obtained from ATP cleavage. DNA ligase is a type of ligase which catalyzes the formation of phosphodiester bond between two nucleotides.

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Kerala Public Service Commission (PSC) Botany Lecturer / Assistant Professor Exam 2009 Question Paper Part 4 (MCQ 013)

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Part – 4 (Questions 76 – 100) (MCQ 013)

(76).  A man who is affected with phenylketonuria marries a woman who is heterozygous at that locus. What is the probability that their first child will have phenylketonuria?

a.       1/8
b.      ¼
c.       ½
d.      ¾

(77).  Which of the following statements about heritability are true?

a.       Is a measure of level of gene linkage
b.      Is a measure of inbreeding
c.       Is a measure of proportion of repeated DNA in an organism
d.      Is a measure of the proportion of variation that is due to genetic causes

(78).  Heat shock proteins were originally described as protein produced in response to heat stress. Some are known to act as:

a.       Molecular chaperones that regulate protein folding
b.      Protein-tyrosine kinase protein
c.       Protease that degrade ubiquitone-tagged protein
d.      GTPase activating protein

(79).  The synthesis of glucose from lactate, glycerol, or amino acid is called:

a.       Glycogenolysis
b.      Glycolysis
c.       Lipolysis
d.      Gluconeogenesis

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Botany Lecturer/Assistant Professor Selection by Kerala PSC 2009 Part 3: Cat. NO. 464/2007 (MCQ 012)

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Part – 3 (Questions 51 – 75) (MCQ 012)

(51).  Buffers has the ability to

a.       Change in pH
b.      Resist change in pH
c.       No resist to change in pH
d.      All of the above

(52).  Why is the filamentous body of the slime and water molds considered an example of convergent evolution with the hyphae of true fungi?

a.       Fungi are closely related to the slime and water molds
b.      Body shape reflects phylogenetic relationships among organisms
c.       Filamentous shape is an adaptation for a nutritional mode as a decomposer with absorptive nutrition
d.      Filamentous body shape is evolutionary primitive for all eukaryotes

(53).  The amount of light absorbed by a material is proportional to the concentration of the absorbing solution is referred as:

a.       Beer’s law
b.      Boger-Lambert law
c.       Poiseuille’s law
d.      All of the above

(54).  Lateral roots in higher plants arise from:

a.       Cortex
b.      Pericycle
c.       Epidermis
d.      Endodermis

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Botany Lecturer/Assistant Professor Examination by Kerala PSC 2009 (Cat. No. 464/2007) Part 2 (MCQ 011)

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Part – 2 (Questions 26 – 50) (MCQ 011)

(26).  True statements about retro-transposons include which of the following?

I.     They replicate through an RNA intermediate
II.   They utilize reverse transcriptase for replication
III. They may contain introns

a.       I only
b.      III only
c.       I and II
d.      I, II and III

(27).  The most recent common ancestor of all land plants was most similar to modern-day members of which group?

a.       Brown algae
b.      Red algae
c.       Charophyceae
d.      Cyanobacteria

(28).  The type of classification which is also known as Adansonian classification:

a.       Numerical taxonomy
b.      Chemo taxonomy
c.       Molecular taxonomy
d.      None of the above

(29).  Which of the following is characteristic of alternation of generations in land plants?

a.       Meiosis in sporophytes produces haploid spores
b.      Gametophytes produce spores that develop in to gametes
c.       Either the gametophyte or the sporophyte is unicellular
d.      Haploid sporophyte make haploid spores

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Kerala PSC Botany Lecturer / Assistant Professor Question Paper: Cat. No. 464/2007: Part 1 (MCQ 010)

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Original question paper of Kerala PSC Botany Lecturer/Assistant Professor (Category No. 464/2007) examination conducted by Kerala PSC (Public Service Commission) on 20/06/2009 (Q. Code 196/2009) for the appointment of Botany Lecturer/Assistant Professor in Government Colleges of Kerala under the Directorate of Collegiate Education, Trivandrum, Kerala. Questions are in MCQ (Multiple Choice Questions) format.


Part – 1 (Questions 1 – 25) (MCQ 010)

(1).  Simple collateral vascular bundles are found in :

a.       Equisetum
b.       Lycopodium
c.       Pleopteris
d.       Selaginella

(2).  Sort out the branched alga :

a.       Oedogonium  
b.       Spirogyra
c.       Zygnema
d.       Ectocarpus

(3). P-proteins are found in :

a.       Companion cells
b.      Sieve elements
c.       Pith
d.      Vessels

(4). A mutation in a codon leads to the substitution of one amino acid with another. What is the name for this type of mutation?

a.       Nonsense mutation
b.      Missense mutation
c.       Frame-shift mutation
d.      Promoter mutation

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