KERALA UNIVERSITY Ph.D. ENTRANCE EXAMINATION NOVEMBER 2009 FACULTY OF APPLIED SCIENCES BIOCHEMISTRY Time: 140 Minutes Maximum Marks: 160 Note: Answer any twelve questions from Section B […]
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easybiologyclass tutorials in biochemistry
Titration Curve of a Weak Acid and its pKa (Biochemistry Notes)
What is Titration? Titration is a method to determine the concentration of a dissolved substance (analyte or titrand) in a known volume by reacting it with another substance of known concentration and volume (titrant). The volume of the reactants plays a crucial role in the titration and thus the titration […]
Continue ReadingProton Hopping in Water (Grotthuss Mechanism)
Proton Hopping in water is the process of diffusion of protons (H⁺ ions) through the network of hydrogen-bonded water molecules in the liquid water. Proton hopping is also called as Grotthuss mechanism, named after the discoverer Theodor Grotthuss. The net result of proton hopping is the fast movement of H⁺ […]
Continue ReadingHow to Calculate pH and pKa of a Buffer Using Henderson-Hasselbalch Equation?
How to Calculate pH and pKa of a Buffer using Henderson-Hasselbalch Equation? Henderson-Hasselbalch equation is a numerical expression which relates the pH, pKa and Buffer Action of a buffer. A buffer is a solution which can resist the change in pH. Chemically, a buffer is a solution of equimolar concentration […]
Continue ReadingpH and pKa – Henderson-Hasselbalch Equation Deriving
Henderson-Hasselbalch equation is a simple expression which relates the pH, pKa and the buffer action of a weak acid and its conjugate base. The Henderson-Hasselbalch equation also describes the characteristic shape of the titration curve of any weak acid such as acetic acid, phosphoric acid, or any amino acid. The […]
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