mcq biology

HSST Botany 2012: Kerala PSC Previous Year Question Paper with Answer Key and Explanations – Part 3

green fluorescent protein

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Kerala PSC HSST Botany 2012 Examination
Higher Secondary School Teacher: Junior & Senior
Question Paper Code 40/2012 (Cat. NO. 449/2010)

Original question paper of Kerala PSC HSST Botany Junior / Senior (Higher Secondary School Teacher Botany), Category No. 449/2010) examination conducted by Kerala PSC (Public Service Commission) on 13/04/2002 (Q. Code 40/2012) for the appointment of HSST Botany in Government Higher Secondary Schools of Kerala under the Directorate of Higher Secondary Education, Trivandrum, Govt. of Kerala. Questions are in MCQ (Multiple Choice Questions) format.

Part – 3 (Questions 51 – 75)

51.  GFP stands for:

a.       Green Fluorescent Protein
b.      Gene Finger Printing
c.       Grey Fluorescent Protein
d.      Green Fluorescent Plants

Ans. (a). Green Fluorescent Protein

GFP: It is a 26.9 dDa protein with 238 amino acids originally isolated from a jellyfish namely Aequorea victoria. GFP emit green fluorescent light when hit with light waves of ultraviolet region. The excitation wave length of GFP is 395 nm and the emission wavelength is at 475 nm (bright green). GFP is extensively utilized in molecular biology research for tagging biomolecules especially proteins for quantification, localization, interaction of proteins with other proteins or other cellular components. Martin Chalfie, Osamu Shimomura and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry for their discovery and development of the green fluorescent protein. Many variants of GFP are now available each with characteristic excitation and emission ranges.

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52.  Asafoetida is obtained from which part of Ferula asafetida:

a.       Stem back
b.      Leaf
c.       Root
d.      Fruit

Ans (c). Root

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