biological chemistry

How to Calculate pH and pKa of a Buffer Using Henderson-Hasselbalch Equation?

Henderson Hasselbalch Equation Sample Problems

How to Calculate pH and pKa of a Buffer using Henderson-Hasselbalch Equation?

Henderson-Hasselbalch equation is a numerical expression which relates the pH, pKa and Buffer Action of a buffer. A buffer is a solution which can resist the change in pH. Chemically, a buffer is a solution of equimolar concentration of a weak acid (such as acetic acid – CH3COOH) and its conjugate base (such as acetate ion – CH3COO¯). In the previous post, we have discussed the Titration Curve of a weak acid and the Derivation of Henderson-Hasselbalch Equation. The characteristic shape of the titration curve of a weak acid is also described by the Henderson-Hasselbalch equation. In this chapter we will discuss the methods to calculate the pH or pKa of a buffer using Henderson-Hasselbalch equation using sample problems.

Learn more: Titration Curve of a Weak Acid (Acetic Acid)

Lean more: How to Derive Henderson-Hasselbalch Equation?

Henderson-Hasselbalch Equation is given as:

Hasselbalch equation


pH – the negative logarithm of H⁺ ion concentration in the medium.

pKa – the negative logarithm of Ka of the acid (Ka is the dissociation constant)

Proton acceptor – the ionized or deprotonated acid (example – CH3COO¯).

Proton donor – intact (non-ionized) weak acid (example – CH3COOH).

Let’s see some sample problems and solutions.

Problem-1: A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate the pH of the medium if the pKa of the acetic acid is 4.76.

Continue reading

biological chemistry

pH and pKa – Henderson-Hasselbalch Equation Deriving

ph and pKa Relationship

Henderson–Hasselbalch Equation
How to Derive Henderson Hasselbalch Equation?

Henderson-Hasselbalch equation is a simple expression which relates the pH, pKa and the buffer action of a weak acid and its conjugate base. The Henderson-Hasselbalch equation also describes the characteristic shape of the titration curve of any weak acid such as acetic acid, phosphoric acid, or any amino acid. The titration curve of a weak acid helps to determine the buffering pH which is exhibited around the pKa of that acid. For example, in the case of acetate buffer, the pKa is 4.76. This is the best buffering pH of acetic acid. Besides, at this pH the acetic acid (CH3COOH) and acetate ions (CH3COO¯) will be at equimolar concentration in the solution. This equimolar solution of a weak acid and its conjugate base will resist the change in pH by donating or taking up the H⁺ ions. (pH is the negative logarithm of hydrogen ion concentration in a medium.The pKa is the negative logarithm of Ka. The Ka is the dissociation constant (similar to the equilibrium constant) for the ionization reaction of an acid.)

Learn more: Titration Curve of a Weak Acid (Acetic Acid)

In the present post, we will see the derivation of Henderson-Hasselbalch equation from the ionization reaction of a weak acid. We also discuss the significance of Henderson-Hasselbalch equation.

Continue reading

Molecular Biology Tutorials

Philadelphia Chromosome and Oncogenic BCR ABL Gene Translocation in CML

Philadelphia Translocation

Philadelphia Chromosome (PH)
(Philadelphia Translocation, PH and Chronic Myeloid Leukemia – CML)

Translocation is a Structural Aberration of Chromosome

Translocation is a type of structural aberration of the chromosome where a segment of chromosome gets translocated to another chromosome. There may be two types of translocation based on the nature of the exchange. They are:

(1). Homologous Translocation

(2). Heterologous Translocation

In homologous translocation, the exchange of chromosomal segments occurs between the homologous chromosomes. In heterologous translocation, the chromosomal segments are exchanged between non-homologous chromosomes. The heterologous translocation in most of the cases will be a reciprocal translocation (exchange of segments between chromosomes).

Translocation causes ‘Position Effect’

The translocation of chromosomes leads to a phenomenon in molecular genetics called the ‘Position Effect’. The position effect is the change in the expression pattern of a gene due to its current position in the chromosome. For example, a normally active gene may be converted to an inactive gene when it is translocated into a new position or vice versa.

Continue reading