Biotechnology Eligibility Test Preparation

DBT BET JRF Exam 2017 Original Solved Question Paper with Answer Key (Download PDF)


DBT JRF 2018 Exam Question Paper

Original (original) Previous Year (old) Solved Question Paper of DBT BET JRF 2017 Examination (Department of Biotechnology- Biotechnology Eligibility Test – Junior Research Fellowship) with Answer Key and Explanations as PDF. DBT BET JRF aspirants can download the question paper as single PDF file for your exam preparation. Please feel free to inform us for any mistakes in the answer key provided.

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Biotechnology Lecture Notes

Applications of Restriction Endonuclease Enzymes in Biotechnology (Short Notes)


Restriction enzymes in Genetic Engineering

Applications of Restriction Enzymes
(The Significance / Importance and Uses of Restriction Endonucleases in Biotechnology)

Restriction endonucleases (also called as molecular scissors) are a class of nuclease enzymes which cut the DNA strand at precise locations. They are specific endonuclease enzymes in the cells which first recognize the specific sequence (called restriction sites) within the DNA strand and cleave the phosphodiester backbone of the DNA at specific sites. The Nobel Prize in 1978 (in Physiology and Medicine) was shared by Werner Arbor, Daniel Nathans and Hamilton Smith for the discovery of restriction enzymes and their applications in molecular genetics. Restriction enzymes are now an inevitable tool for the manipulation of DNA in various recombination studies both in vitro and in vivo. The main applications of restriction enzymes are:

(1). Construction of Restriction Maps

(2). Construction of DNA Fingerprints

(3). Recombinant DNA Technology (rDNA Technology)

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mcq biology

DBT BET JRF Exam 2017 Model Questions with Answer Key and Explanations Part 1


dbt bet 2017 free online test

image source wikipedia

DBT BET JRF Exam, 2017
Model Question Paper with Answer Key and Explanations

(Biology / Life Sciences MCQ: Multiple Choice Questions)

(1).  _____ can serve as an alternative to ethidium bromide to stain DNA for detection on gels

a.       Mitomycin C
b.      Acridine orange
c.       SYBR Green I
d.      Acriflavine

(2). _____ is a sensitive technique to find out the number of template molecules originally present in a PCR reaction.

a.       Hot start PCR
b.      Real time PCR
c.       AP PCR
d.      Reverse Transcriptase PCR

(3).  Using __________ it is possible to generate fluorescence in quantitative PCR reactions

a.       TaqMan probes
b.      Molecular beacons
c.       Scorpion probes
d.      All of the above

(4). For whole genome amplification, creating very long DNA products ________ method can be employed with great success

a.       MDA
b.      PEP
c.       iPEP
d.      DOP

(5).  ________ polymerase has the ability to add ~70,000 nucleotides every time it binds to primer and has a very low error rate.

a.       Taq polymerase
b.      Tli polymerase
c.       Pfu polymerase
d.      Bacteriophage ϕ 29 polymerase

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Biotechnology Lecture Notes

Batch Fermentation vs Continuous Fermentation Process: Similarities and Differences – A Comparison Table


Difference continuous and batch fermentation

Batch Fermentation vs Continuous Fermentation Process
(Similarities and difference between Batch Fermentation and Continuous Fermentation Process)

Batch Fermentation and Continuous Fermentation are the two commonly adopted Industrial Fermentation methods for the scale production of microbial biomass or metabolites.

Batch Fermentation: Here the fermenter is first filled with the raw material (carbon source). Then the microbes are added and allowed to ferment the raw material under optimum pH and aeration. The products remain in the fermenter until the completion of fermentation. After fermentation, the products are extracted and the fermenter is cleaned and sterilized before next round. Thus here the fermentation is done as separate batches.

Continuous Fermentation: Here the exponential growth rate of the microbes is maintained in the fermenter for prolonged periods of time in by the addition of fresh media are regular intervals. The metabolite or the product of fermentation is extracted for the overflow from the fermenter. Thus unlike batch fermentation, in continuous fermentation, the fermentation process never stops in between and it continues to run for a long period of time with the addition of nutrients and harvesting the metabolites at regular intervals.

The present post describes the Similarities and Differences between Batch Fermentation and Continuous Fermentation Process as a Comparison Table.

Similarities between Batch Culture and Continuous Culture Fermentation Methods

Ø  Both are industrial fermentation methods for the large scale production.

Ø  Both methods can be used for the production of microbial biomass or products.

Ø  Both run under controlled environmental conditions

Ø  The mechanical components of fermenter is almost similar in both types




Difference between Batch Fermentation and Continuous Fermentation Process

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how to qualify IIT JAM BT?

IIT JAM BT 2006 (Biotechnology) Solved Question Paper Original (Download PDF)


JAM BT 2006 Original Question Paper

Official / Original Previous Year (Old) Solved Question Paper of IIT / IISc Joint Admission Test for MSc JAM Biotechnology (BT) Examination 2006 with Answer Key and Explanations as PDF. JAM aspirants can download the question paper as single PDF file for your exam preparation. Please feel free to inform us for any mistakes in the answer key provided.

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