Original (original) Previous Year (old) Solved Question Paper of DBT BET JRF 2017 Examination (Department of Biotechnology- Biotechnology Eligibility Test – Junior Research Fellowship) with Answer Key and Explanations as PDF. DBT BET JRF aspirants can download the question paper as single PDF file for your exam preparation. Please feel free to inform us for any mistakes in the answer key provided.
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Applications of Restriction Enzymes (The Significance / Importance and Uses of Restriction Endonucleases in Biotechnology)
Restriction endonucleases (also called as molecular scissors) are a class of nuclease enzymes which cut the DNA strand at precise locations. They are specific endonuclease enzymes in the cells which first recognize the specific sequence (called restriction sites) within the DNA strand and cleave the phosphodiester backbone of the DNA at specific sites. The Nobel Prize in 1978 (in Physiology and Medicine) was shared by Werner Arbor, Daniel Nathans and Hamilton Smith for the discovery of restriction enzymes and their applications in molecular genetics. Restriction enzymes are now an inevitable tool for the manipulation of DNA in various recombination studies both in vitro and in vivo. The main applications of restriction enzymes are:
Batch Fermentation vs Continuous Fermentation Process (Similarities and difference between Batch Fermentation and Continuous Fermentation Process)
Batch Fermentation and Continuous Fermentation are the two commonly adopted Industrial Fermentation methods for the scale production of microbial biomass or metabolites.
Batch Fermentation: Here the fermenter is first filled with the raw material (carbon source). Then the microbes are added and allowed to ferment the raw material under optimum pH and aeration. The products remain in the fermenter until the completion of fermentation. After fermentation, the products are extracted and the fermenter is cleaned and sterilized before next round. Thus here the fermentation is done as separate batches.
Continuous Fermentation: Here the exponential growth rate of the microbes is maintained in the fermenter for prolonged periods of time in by the addition of fresh media are regular intervals. The metabolite or the product of fermentation is extracted for the overflow from the fermenter. Thus unlike batch fermentation, in continuous fermentation, the fermentation process never stops in between and it continues to run for a long period of time with the addition of nutrients and harvesting the metabolites at regular intervals.
The present post describes the Similarities and Differences between Batch Fermentation and Continuous Fermentation Process as a Comparison Table.
Similarities between Batch Culture and Continuous Culture Fermentation Methods
Ø Both are industrial fermentation methods for the large scale production.
Ø Both methods can be used for the production of microbial biomass or products.
Ø Both run under controlled environmental conditions
Ø The mechanical components of fermenter is almost similar in both types
Difference between Batch Fermentation and Continuous Fermentation Process
Official / Original Previous Year (Old) Solved Question Paper of IIT / IISc Joint Admission Test for MSc JAM Biotechnology (BT) Examination 2006 with Answer Key and Explanations as PDF. JAM aspirants can download the question paper as single PDF file for your exam preparation. Please feel free to inform us for any mistakes in the answer key provided.
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