Difference between Exonuclease and Endonuclease: Comparison Table

Endonuclease vs Exonuclease

Endonuclease vs Exonuclease
(Similarities and Differences between Endonuclease and Exonuclease Enzyme)

Nucleases are a class of enzyme which hydrolyzes the nucleic acids such as DNA and RNA.  They hydrolyze the phosphodiester backbone which connects individual nucleotides in a polynucleotide. There are two broad categories of nucleases depending upon their site and mode of action on the nucleic acid. They are Endonucleases and Exonucleases.

Endonucleases: Endonucleases cleaves the phosphodiester bond in the polynucleotide from the interior (endo). They hydrolyze the phosphodiester bonds present within a polynucleotide chain.  Some endonucleases are non-specific and they can cut the phosphodiester bond between any nucleotides. A special class of endonucleases, called restriction endonucleases (restriction enzymes), is very specific in their action and they cut at a specific sequence in the polynucleotide chain called the Restriction Site. Restriction endonuclease has immense application in recombinant DNA technology.

Learn more: Applications of Restriction Enzymes

Exonucleases: They are nuclease enzyme which cleaves the nucleotides from the ends. They hydrolyze the phosphodiester bonds present either at 3’ or 5’ end of the polynucleotide chain.

The present post discusses the similarities and differences between endonucleases and exonucleases with a comparison table.

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biological chemistry

Difference between Collagen and Keratin – Comparison Table

Collagen vs Keratin

Collagen vs Keratin
(Similarities and Differences between Collagen and Keratin)

Collagen and Keratin are the main structural proteins. They are two main families of fibrous proteins and are abundantly present in the extracellular matrix and various connective tissues in animals. 

Collagen: A linear structural fibrous protein abundantly found in the connective tissue and extracellular space of the cells. They are also found in tendons, ligaments, cornea, cartilage, bone, blood vessels, intervertebral discs and the dentine in teeth.

Keratin: A structural protein abundantly present in epithelial cells. They protect the epithelial cells from damage or stress. Keratin is also present in appendages such as feathers, hairs and nail, horn, claws and hooves of animals. Keratin is the structural material that makes the human skin.

The present post discusses the similarities and Differences between Collagen and Keratin with a Comparison Table.

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Botany lecture notes

Difference between Intercropping and Mixed Cropping: Comparison Table

Intercropping vs Mixed Farming

Mixed Cropping vs Intercropping
(Difference between Mixed Cropping and Intercropping)

Mixed cropping and Intercropping are two methods of diversified farming techniques where more than one types of crops are grown in the same unit area. In mixed cropping or mixed farming, two independent crops are mixed together and grown in an area, whereas the intercropping is a multiple cropping techniques where two or more crops are grown in proximity.  Mixed cropping and intercropping are essentially two separate farming techniques with specific goals. The present post discusses the differences between Mixed cropping and Intercropping with a comparison table.

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