Advantages and Disadvantages of Cell / Tissue Culture
Tissue culture is an aseptic in vitro culture of animal or plant cells in a precisely controlled environmental condition. The term “Tissue Culture” was for the first time coined by Thomas Burrows. Even though the in vitro culture of prokaryotes and both plant and animal cells are possible now, the term ‘Tissue Culture’ generally denotes to Animal Cell / Tissue culture. The term ‘Plant Tissue Culture’ symbolizes the in vitro culture of plant cells.
In cell culture the cells are maintained in an artificial environment consists of a suitable culture vessel containing a culture medium. The medium supplies all the nutrients, growth factors, and required gases for the growth of the cells. Moreover, the physiochemical environment of the culture system is strictly monitored most probably through automated systems. The control of the physiochemical environment in the culture system is one of the most important advantages of cell culture system. However, the tissue culture process is not always profitable and there are a plenty of difficulties in maintaining the cells in in vitro conditions. The present post describes the Advantages and Disadvantages of animal cell or tissue culture techniques.
Advantages of Animal Cell Culture:
Ø Physio-chemical environment in the culture such as pH, temperature, osmolarity and level of dissolved gases can be precisely controlled in the in vitro system.