Phase Contrast Microscopy PPT by Easybiologyclas

Biophysical Instrumentation PPT

Phase Contrast Microscopy PPT

What is Phase Contrast Microscope? What are the limitations of ordinary light microscope? Who invented the phase contrast microscope? What is the working principle of phase contrast microscope? What are the optical components of phase contrast microscope? What is the importance of Annular Diaphragm and Phase Plate in phase contrast microscope? How contrast is created in phase contrast microscopy? What are the applications of phase contrast microscope? What are the limitations of phase contrast microscopy?

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Phase Contrast Microscopy – Optical Components, Working Principle and Applications (Short Notes with PPT)

Applications of phase contrast microscope

Phase Contrast Microscopy
(Optical Components, Working Principle and Applications of Phase Contrast Microscope)

Working Principle of an Ordinary Microscope:

contrast in light microscopyIn an ordinary microscope, the object is viewed due to differences in colour intensities of the specimen. To create the colour intensities, the specimen is first stained with suitable dyes which will impart specific colour. In an ordinary microscope, the contrast is obtained when the light rays pass through a stained specimen because different stains absorb different amounts of light. These differential absorption properties of stained specimen modify the intensity or amplitude of the light waves transmitted by different regions of the cells and this ultimately creates contrast in the image. Thus, staining is essential to create contrast in an ordinary microscope. Moreover, the unstained specimen cannot be observed through an ordinary microscope.

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Why Phase Contrast Microscope?

The Phase Contrast Microscope is used to visualize unstained living cells. Most of the stains or staining procedures will kill the cells.  Phase contrast microscopy enables the visualization of living cells and life events.

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